Gameplay is based around multiple mini-games, each quite simple and straightforward to play using the four Buzz! controllers. The game primarily aimed at the family market but offers appealing entertainment to almost anyone of any age. Simple game play allows young children to participate while still being entertaining enough for older children and adults.

The game is similar in concept to the other Buzz! Junior-games, offering dinosaur-based mini-games in a prehistoric environment. One of the main new additions Dino Den brings to the series is team-play, where the 4 players are divided into 2 teams and work together to win that specific mini-game. If there are not enough human players, a computer player will fill the open slot.